Diversity of Escherichia coli O157 in a longitudinal farm study using multiple-locus variable-number tandem-repeat analysis.
作者： A.M. Urdahl , N.J.C. Strachan , Y. Wasteson , M. MacRae , I.D. Ogden
DOI: 10.1111/J.1365-2672.2008.03856.X
关键词:
摘要: Aims: To perform a longitudinal study of the diversity Escherichia coli O157 from ruminant pasture/stream environment using multiple-locus variable-number tandem-repeat analysis (MLVA). Methods and Results: Samples faecal droppings grazing ruminants an adjacent stream were tested longitudinally for E. by enrichment immunomagnetic separation (IMS). Using MLVA, 24 different profiles identified total 231 isolates, which 80 included in similarity analysis. Four main clusters with several subclusters observed. Although there was close contact between sheep cattle during period, surprisingly not detected faeces. Conclusions: The cluster indicated both unrelated closely related strains. choice loci to target MLVA is important subtyping result, as high diversities are essential discriminating isolates. Significance Impact Study: There lack data available on use describe changes over time animal reservoirs environment. Such needed order further develop typing method.
elsevier.com
sci-hub.se 参考文章(41)
D.A. McDowell, J. J. Sheridan, Survival and Growth of VTEC in the Environment Food & Nutrition Press, Inc.. pp. 279- 304 ,(2008) , 10.1002/9780470385098.CH15
Jorge Blanco, Miguel Blanco, Jesus E. Blanco, Azucena Mora, Mara Pilar Alonso, Enrique A. Gonzalez, Mara Isabel Bernardez, Epidemiology of Verocytotoxigenic Escherichia coli (VTEC) in Ruminants Verocytotoxigenic E. coli. pp. 113- 148 ,(2008) , 10.1002/9780470385098.CH6
Bjørn-Arne Lindstedt, Traute Vardund, Georg Kapperud, Multiple-Locus Variable-Number Tandem-Repeats Analysis of Escherichia coli O157 using PCR multiplexing and multi-colored capillary electrophoresis. Journal of Microbiological Methods. ,vol. 58, pp. 213- 222 ,(2004) , 10.1016/J.MIMET.2004.03.016
S.M. Avery, A. Moore, M.L. Hutchison, Fate of Escherichia coli originating from livestock faeces deposited directly onto pasture. Letters in Applied Microbiology. ,vol. 38, pp. 355- 359 ,(2004) , 10.1111/J.1472-765X.2004.01501.X
A. Maule, Survival of verocytotoxigenic Escherichia coli O157 in soil, water and on surfaces. Journal of Applied Microbiology. ,vol. 88, ,(2000) , 10.1111/J.1365-2672.2000.TB05334.X
Eija Hyytiä-Trees, Sandra C. Smole, Patricia A. Fields, Bala Swaminathan, Efrain M. Ribot, Second Generation Subtyping: A Proposed PulseNet Protocol for Multiple-Locus Variable-Number Tandem Repeat Analysis of Shiga Toxin–ProducingEscherichia coliO157 (STEC O157) Foodborne Pathogens and Disease. ,vol. 3, pp. 118- 131 ,(2006) , 10.1089/FPD.2006.3.118
Carol Myers, Christopher Rose, Christine Keys, Jeff Farrar, Robert E. Mandrell, Michael Cooley, Diana Carychao, Leta Crawford-Miksza, Michele T. Jay, Incidence and Tracking of Escherichia coli O157:H7 in a Major Produce Production Region in California PLOS ONE. ,vol. 2, ,(2007) , 10.1371/JOURNAL.PONE.0001159
C. Keys, S. Kemper, P. Keim, Highly diverse variable number tandem repeat loci in the E. coli O157:H7 and O55:H7 genomes for high-resolution molecular typing. Journal of Applied Microbiology. ,vol. 98, pp. 928- 940 ,(2005) , 10.1111/J.1365-2672.2004.02532.X
J. T. LeJeune, T. E. Besser, D. H. Rice, J. L. Berg, R. P. Stilborn, D. D. Hancock, Longitudinal Study of Fecal Shedding of Escherichia coli O157:H7 in Feedlot Cattle: Predominance and Persistence of Specific Clonal Types despite Massive Cattle Population Turnover Applied and Environmental Microbiology. ,vol. 70, pp. 377- 384 ,(2004) , 10.1128/AEM.70.1.377-384.2004
D. A. WIDIASIH, N. IDO, K. OMOE, S. SUGII, K. SHINAGAWA, Duration and magnitude of faecal shedding of Shiga toxin-producing Escherichia coli from naturally infected cattle. Epidemiology and Infection. ,vol. 132, pp. 67- 75 ,(2004) , 10.1017/S0950268803001468