Hemoglobin F synthesis in vitro: evidence for control at the level of primitive erythroid stem cells

摘要: The in vitro regulation of fetal hemoglobin (HbF was investigated clones cultured adult human erythroid cells by situ immunofluorescent identification the hemoglobins synthesized. Formation Hb F-containing enhanced erythropoietin and culture conditions favoring proliferation less-differentiated stem burst-forming-unit type. Burst-forming units differed their capacity to direct F synthesis terminally differentiated progeny. A class early precursors that can produce descendent with or without commitment production identified. findings suggest capability for expression is determined at level characteristics burst-forming units. It proposed vivo also linked process differentiation patterns during ontogeny reflect attainment progressively higher levels as development proceeds.