Sequence selective binding of bis-daunorubicin WP631 to DNA.

摘要: We have used footprinting techniques on a wide range of natural and synthetic substrates to examine the sequence-selective interaction bis-daunorubicin antibiotic WP631 with DNA. The ligand produces clear DNase I footprints that are very different from those seen other anthracycline antibiotics such as daunorubicin nogalamycin. Footprints found in diverse sequences, many which rich GT (AC) or GA (TC) residues. As expected, binds well sequences CGTACG CGATCG, but also at hexanucleotide GCATGC GCTAGC. various do not contain any particular unique di-, tri- tetranucleotide frequently sequence (G/C)(A/T)(A/T)(G/C). All this composition protected by ligand, though it can bind some sites differ consensus one base pair.