Phyllosphere bacterial communities of trichome-bearing and trichomeless Arabidopsis thaliana leaves

摘要: This study aimed to investigate whether the presence of trichomes as conspicuous physical attributes leaf surface affects microbial community composition on Arabidopsis thaliana leaves. The A. ecotype Col-0 and its trichomeless gl1 mutant were grown in growth cabinets under climate-controlled conditions. showed a similar wax wild type with slightly reduced amounts C29, C31 C33 alkanes by GC/MS GC/FID analyses. 120 bacterial isolates representing 39 genera obtained from surfaces. Phylogenetic analysis nearly full-length 16S rRNA sequences 29 selected confirmed their affiliation Proteobacteria (Alpha-, Beta-, Gamma-), Actinobacteria, Bacteroidetes Firmicutes. diversity mutant, devoid trichomes, further compared denaturing gradient gel electrophoresis (DGGE). Banding patterns sequencing representative DGGE bands revealed phylotypes related Sphingomonas (Alphaproteobacteria), Methylophilus (Betaproteobacteria) Dyadobacter (Bacteroidetes) which are common phyllosphere inhabitants. Furthermore, wildtype plants exposed outdoor conditions for 4–5 weeks. gels only minor differences between two plant lines, thus suggesting that per se do not affect leaves experimental tested.