ZnO Nanoparticles Affect Bacillus subtilis Cell Growth and Biofilm Formation

摘要: Zinc oxide nanoparticles (ZnO NPs) are an important antimicrobial additive in many industrial applications. However, mass-produced ZnO NPs ultimately disposed of the environment, which can threaten soil-dwelling microorganisms that play roles biodegradation, nutrient recycling, plant protection, and ecological balance. This study sought to understand how affect Bacillus subtilis, a plant-beneficial bacterium ubiquitously found soil. The impact on B. subtilis growth, FtsZ ring formation, cytosolic protein activity, biofilm formation were assessed, our results show growth is inhibited by high concentrations (≥ 50 ppm), with cells exhibiting prolonged lag phase delayed medial formation. RedoxSensor Phag-GFP fluorescence data further at ZnO-NP above ppm, reductase membrane stability, expression all decrease. SDS-PAGE Stains-All staining FT-IR demonstrate negatively exopolysaccharide production. Moreover, it was surface structures became smooth under only 5–10 ≤ 25 ppm significantly reducing activity. XANES EXAFS spectra analysis confirmed presence co-cultured cells, suggests penetration cell membranes either or toxic Zn+ ions from ionized NPs, latter may be deionized within bacterial cells. Together, these viability through inhibition expression, suggest future waste management strategies would do well mitigate potential environmental engendered disposal nanoparticles.