Transmitochondrial Technology in Animal Cells

摘要: Publisher Summary This chapter describes the current protocols used to generate transmitochondrial animal cell lines in culture. Studies of vertebrate mitochondrial DNA (mtDNA) maintenance and function have relied heavily on somatic experimentation culture, because one is still unable manipulate mtDNA sequences at will cells. Initial patterns segregation species-specific compatibility were performed using hybrid cybrid Cybrids, or cytoplasmatic hybrids, are eukaryotic produced by fusion a nuclear donor mitochondria from donor. Therefore, formed genome source genomes different one. As result, this model, it possible dissociate biochemical influence its background. Hybrid cells may be several techniques, including fusion. Different tissue types techniques been employed for generation animals. Basically, line devoid with poisoned works as donor, while enucleated fragmented On membrane fusions appropriate selection, generated. Although manipulating heteroplasmy mtDNA-targeted restriction endonucleases (RE) can powerful approach modulate heteroplasmy, limited availability unique RE cleavage site. The also useful context multiple sites, if expression targeted could controlled.