Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies
作者： Julie Welburn , Jane Endicott
DOI: 10.1385/1-59259-857-9:219
关键词:
摘要: The determination of structures for proteins that control the eukaryotic cell cycle by nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography has made a significant contribution to our understanding molecular mechanisms progression. CDK2 proved particularly tractable structural analysis, in complex with various regulatory different phosphorylation states provides paradigm this important kinase family. This chapter describes number protocols can be used prepare CDKs selected CDK binding suitable studies heterologous expression either E. coli or insect cells.
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