Confocal and multiphoton imaging of intracellular Ca(2

作者: Godfrey Smith , Martyn Reynolds , Francis Burton , Ole Johan Kemi , None

DOI: 10.1016/B978-0-12-374841-6.00009-8

关键词:

摘要: This chapter compares the imaging capabilities of a range systems including multiphoton microscopy in regard to measurements intracellular Ca(2+) within living cells. In particular, excitation spectra popular fluorescent indicators are shown during 1P and 2P excitation. The strengths limitations current discussed along with error analysis which highlights value matching affinity dye particular aspect signaling. Finally, combined emission voltage sensitive dyes compared allow choice optimum combination simultaneous membrane measurement.

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