Genome-wide analysis of sixteen chordomas by comparative genomic hybridization and cytogenetics of the first human chordoma cell line, U-CH1

摘要: Cytogenetic information on chordomas is rudimentary and restricted to GTG-banding analysis of 26 cases worldwide. In this study, we present the chromosomal imbalances detected in a series 16 (10 sacrococcyeal, five sphenooccipital, one spinal) from 13 patients using comparative genomic hybridization (CGH) fluorescence situ (FISH). On average, 3.2 losses 4.2 gains were per tumor. The most common DNA copy number alterations arms 3p (50%) 1p (44%). Losses seven primary chordomas. Therefore, loss might be an early event chordoma genesis. involved 7q (69%), 20 (50%), 5q (38%), 12q (38%). Additionally, raised first human cell line, U-CH1, recurrence sacral chordoma. U-CH1 its parent tumor had almost same CGH profile. According multicolor FISH, has following clonal abnormalities: der(1)t(1;22), del(4), +del(5), +del(6), +7, del(9), del(10), +der(20)t(10;20), +21. Thus, novel permanent line chordoma-typical cytogenetic aberrations. Our data suggest that suppressor genes or mismatch repair (located at 1p31 3p14) oncogenes 7q36)