Identification of a testis-specific gene from the mouse t-complex next to a CpG-rich island.

摘要: Abstract We have used an approach based on the observation of CpG-rich regions near 5' end many genes to screen a panel cosmids derived from t-complex and tested candidate sequences for evidence transcription in number different mouse tissues. One gene so identified is expressed specifically testicular germ cells maps subregion also containing loci involved transmission ratio distortion male sterility. The transcript first detected during pachytene stage meiotic division, but highest levels later haploid spermatogenic stages. Sequence analysis verified existence element predicts unique protein species with no significant homologies those previously determined.