Nanomaterial based self-referencing microbiosensors for cell and tissue physiology research.

摘要: Physiological studies require sensitive tools to directly quantify transport kinetics in the cell/tissue spatial domain under physiological conditions. Although biosensors are capable of measuring concentration, their applications limited due relatively low sensitivity, excessive drift/noise, and inability analyte transport. Nanomaterials significantly improve electrochemical transduction microelectrodes, make construction highly microbiosensors possible. Furthermore, a novel biosensor modality, self-referencing (SR), enables direct measurement real-time flux drift/noise subtraction. SR based on nanomaterials have been used measure several coupled with various stimulators/inhibitors. These include: glucose uptake pancreatic β cells, cancer muscle tissues, intestinal tissues P. Aeruginosa biofilms; glutamate near neuronal cells; endogenous indole-3-acetic acid surface Zea mays roots. Results from provide important insights into cancer, diabetes, nutrition, neurophysiology, environmental plant physiology dynamic conditions, demonstrating that an extremely valuable tool for research.