Single-pulsed electromagnetic field therapy increases osteogenic differentiation through Wnt signaling pathway and sclerostin downregulation

摘要: Pulsed electromagnetic field (PEMF) therapy has been used for more than three decades to treat bone diseases. The main complaint about using PEMF is that it time-consuming. Previously, we showed single-pulsed (SPEMF) applied 3 min daily increased osteogenic differentiation of mesenchymal stem cells and accelerated growth in a long defect model. In the current study, investigated mechanism SPEMF increase osteoblastic cells. We found both short-term (SS) long-term (SL) treatment mineralization, while alkaline phosphatase (ALP) activity during first 5 days treatment. SS gene expression Wnt1, Wnt3a, Wnt10b, Fzd9, ALP, Bmp2. Also, inhibited sclerostin after treatment, inhibition was significant with SL parathyroid hormone-related protein (PTHrP) but decreased Sost gene, which encodes sclerostin. Together, early effect utilizes canonical Wnt signaling pathway inhibitory on may be attributable PTHrP upregulation. This study enhances our understanding cellular mechanisms support previous finding provide new insight clinical applications.