Detection of the Neu5 Ac (alpha 2,3) Gal (beta 1,4) GlcNAc sequence with the leukoagglutinin from Maackia amurensis: light and electron microscopic demonstration of differential tissue expression of terminal sialic acid in alpha 2,3- and alpha 2,6-linkage.

摘要: The Maackia amurensis leukoagglutinin has been shown to react specifically with the Neu5Ac (alpha 2,3) Gal sequence of asparagine-linked complex type oligosaccharides. We report here preparation lectin-gold complexes and their application for light electron microscopic detection Neu5 Ac in various tissues. use lectin directly gold labeled was superior a two-step cytochemical affinity technique using fetuin-gold complex. staining inhibited by pre-incubation 50 mM alpha 2,3 sialyllactose, whereas 2,6 sialyllactose up concentrations 1 M had no effect, thus demonstrating high specificity histochemical staining. In addition N-glycanase-sensitive oligosaccharides, beta-elimination-sensitive serine/threonine-linked oligosaccharides could be detected. Data are presented which show that cellular patterns obtained may differ from those elderberry bark lectin-gold, detects 2,6) Gal/GalN sequence. Electron double labeling direct study differential distribution sequences is reported. Therefore, availability two sialic acid binding lectins different linkage histochemistry provides first opportunity tissue cell expression these terminal glycoproteins.