Chondroitin sulfate facilitates an acrosome reaction in bovine spermatozoa as evidenced by light microscopy, electron microscopy and in vitro fertilization.

摘要: Bovine epididymal spermatozoa were incubated for 22 h in a modified Tyrode's medium. The percentages of sperm exhibiting an acrosome reaction determined morphologically after fixing and staining specimens. addition chondroitin sulfate A (CS-A) significantly increased the incidence reaction. When observed by electron microscopy, acrosome-reacted had undergone vesiculation plasma outer acrosomal membranes. Sperm presence CS-A demonstrated higher when compared to controls. Additionally, rates vitro fertilization bovine oocytes elevated ova exposed CS-A. These results suggest that glycosaminoglycans female reproductive tract may be responsible some biochemical changes associated with fertilization, light microscope procedure can used assess occurrence