Photoclickable dendritic molecular glue: noncovalent-to-covalent photochemical transformation of protein hybrids.
作者： Noriyuki Uchida , Kou Okuro , Yamato Niitani , Xiao Ling , Takayuki Ariga
DOI: 10.1021/JA401059W
关键词:
摘要: A water-soluble dendron with a fluorescein isothiocyanate (FITC) fluorescent label and bearing nine pendant guanidinium ion (Gu+)/benzophenone (BP) pairs at its periphery (GlueBP-FITC) serves as “photoclickable molecular glue”. By multivalent salt-bridge formation between Gu+ ions oxyanions, GlueBP-FITC temporarily adheres to kinesin/microtubule hybrid. Upon subsequent exposure UV light, this noncovalent binding is made permanent via cross-linking reaction mediated by carbon radicals derived from the photoexcited BP units. This temporal-to-permanent transformation light occurs quickly efficiently in preorganized state, allowing movements of microtubules on kinesin-coated glass plate be photochemically controlled. fundamental difference such temporal bindings was visualized use “optical tweezers”.
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sci-hub.se 参考文章(32)
Amy L. Cilia La Corte, Helen Philippou, Robert A.S. Ariëns, Role of Fibrin Structure in Thrombosis and Vascular Disease Advances in Protein Chemistry. ,vol. 83, pp. 75- 127 ,(2011) , 10.1016/B978-0-12-381262-9.00003-3
Bleecker Springs, Paul Haake, Equilibrium constants for association of guanidinium and ammonium ions with oxyanions: The effect of changing basicity of the oxyanion Bioorganic Chemistry. ,vol. 6, pp. 181- 190 ,(1977) , 10.1016/0045-2068(77)90019-0
Ahmet Yildiz, Michio Tomishige, Ronald D Vale, Paul R Selvin, Kinesin Walks Hand-Over-Hand Science. ,vol. 303, pp. 676- 678 ,(2004) , 10.1126/SCIENCE.1093753
Juliane P. Caviston, Erika L.F. Holzbaur, Microtubule motors at the intersection of trafficking and transport Trends in Cell Biology. ,vol. 16, pp. 530- 537 ,(2006) , 10.1016/J.TCB.2006.08.002
Yushi Suzuki, Kou Okuro, Tadashi Takeuchi, Takuzo Aida, Friction-Mediated Dynamic Disordering of Phospholipid Membrane by Mechanical Motions of Photoresponsive Molecular Glue: Activation of Ion Permeation Journal of the American Chemical Society. ,vol. 134, pp. 15273- 15276 ,(2012) , 10.1021/JA3074424
D. D. Hackney, Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 6865- 6869 ,(1994) , 10.1073/PNAS.91.15.6865
Juan Sánchez-Cortés, Milan Mrksich, The Platelet Integrin αIIbβ3 Binds to the RGD and AGD Motifs in Fibrinogen Chemistry & Biology. ,vol. 16, pp. 990- 1000 ,(2009) , 10.1016/J.CHEMBIOL.2009.08.012
Samuel Schmidt, Peter Friedl, Interstitial cell migration: integrin-dependent and alternative adhesion mechanisms Cell and Tissue Research. ,vol. 339, pp. 83- 92 ,(2010) , 10.1007/S00441-009-0892-9
Praveen C Ashok, Kishan Dholakia, Optical trapping for analytical biotechnology. Current Opinion in Biotechnology. ,vol. 23, pp. 16- 21 ,(2012) , 10.1016/J.COPBIO.2011.11.011
Kou Okuro, Kazushi Kinbara, Kouhei Tsumoto, Noriyuki Ishii, Takuzo Aida, Molecular glues carrying multiple guanidinium ion pendants via an oligoether spacer: stabilization of microtubules against depolymerization. Journal of the American Chemical Society. ,vol. 131, pp. 1626- 1627 ,(2009) , 10.1021/JA800491V