Sip, an integrase protein with excision, circularization and integration activities, defines a new family of mobile Staphylococcus aureus pathogenicity islands.
作者： Carles Úbeda , Ma. Ángeles Tormo , Carme Cucarella , Pilar Trotonda , Timothy J. Foster
DOI: 10.1046/J.1365-2958.2003.03577.X
关键词:
摘要: We report the complete sequence of Staphylococcal pathogenicity island bovine 2 (SaPIbov2), encoding biofilm-associated protein Bap. SaPIbov2 contains 24 open reading frames, including sip, which encodes a functional staphylococcal integrase protein. is bordered by 18 bp direct repeats. The integration site into chromosome lies at 3' end gene GMP synthase. has extensive similarity to previously described islands Staphylococcus aureus. principal difference that toxin genes present in other are exchanged for transposon-like element carries bap and an ABC transporter transposase. Also, can be excised form circular integrate site-specifically RecA-independently chromosomal att Sip-dependent manner. This was demonstrated both S. aureus with plasmid substrates ectopically Escherichia coli. Thus, recombinase family promotes excision insertion/integration. In addition, we presence facilitated persistence intramammary gland infection model. Finally, different isolates were found carry related SaPIbov2, suggesting existence islands.
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