DEVELOPMENT OF METHODS FOR THE LARGE-SCALE CULTURE OF OXYTRICHA NOVA

摘要: Publisher Summary Hypotrichous ciliates, such as Oxytricha nova, have two types of nuclei: transcriptionally inactive micronuclei and active macronuclei. The micronucleus has chromosome-size DNA molecules divides mitotically. macronucleus gene-size fragments amitotically. This chapter discusses the development methods for large-scale culture nova. nova can be grown in specially designed 50-liter fermentation vats with very gentle agitation aeration. Chlorogonium are to a Klett 90–100 units 48 liters steril Oochromonas medium . Then, mature cultures added. at 25°C under fluorescent lights. Within seven days, consume almost all chlorogonium reach steady-state density about 8,000 cells/ml. cell distribution is uniform. Using this cell-culture method, quantities macronuclei sufficient comparative biochemical biophysical analyses chromatin isolated.