Cloning of rat nephrin: Expression in developing glomeruli and in proteinuric states

摘要: Cloning of rat nephrin: Expression in developing glomeruli and proteinuric states. Background Nephrin is identified as a product the gene mutated patient with congenital nephrotic syndrome Finnish type. However, its precise localization function are not yet fully clarified. Methods To clone homologue nephrin, polymerase chain reaction (PCR) was employed. elucidate expression immunohistological analysis specific antirat nephrin antibody, reverse transcription-PCR, RNase protection assay were performed. Results Amino acid sequences human highly homologous (82.2% identity). The domain structure also conserved between rats humans. detected only kidney along glomerular capillary walls, always identical to that anti-slit diaphragm monoclonal antibody (mAb) 5-1-6–recognized antigen normal matured fetal staining pattern clearly distinguished from zonula occludens-1 (ZO-1), α3-integrin, or podocalyxin. mRNA for first kidneys at 18.5 embryonic days. decreased just after injection mAb 5-1-6 (47.4%) puromycin aminonucleoside (51.2%), shifted linear granular both Conclusions localized slit antigen. involved development proteinuria nephropathy, but nephropathy.