Molecular characterization of haemoparasites infecting bats (Microchiroptera) in Cornwall, UK.
作者： R. CONCANNON , K. WYNN-OWEN , V. R. SIMPSON , R. J. BIRTLES
DOI: 10.1017/S0031182005008097
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摘要: The presence of haemoparasites from the Order Piroplasmida and genera Bartonella Trypanosoma was assessed in blood 60 bats, belonging to 7 species, inhabiting sites across Cornwall southwest England. DNA extracted macerated heart tissue incorporated into taxon-specific polymerase chain reactions (PCRs) amplification products were sequenced as a means identifying, or assigning an identity, detected haemoparasites. A species 6 Pipistrellus spp., whereas infections 5 bats 4 different species. dionisii 1 spp. Phylogenetic inference alignment partial 18S rRNA-encoding gene sequence pipistrelle-associated with homologous sequences available for other members indicated that this organism unique but specifically related genus Babesia, phylogeny would be keeping being Babesia vesperuginis. Alignment citrate synthase bat-associated bartonellae revealed distinct genotypes probably derived 2 study demonstrates utility molecular methods detecting dead provides, first time, tangible identities
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