Profiling of the Differential Abundance of Drought and Salt Stress-Responsive MicroRNAs Across Grass Crop and Genetic Model Plant Species

摘要: In recent years, it has become readily accepted among interdisciplinary agriculturalists that the current global crop yield to land capability ratio is significantly insufficient achieve food security for predicted population of 9.5 billion individuals by year 2050. This issue further compounded the: (1) versus biofuel debate; (2) decreasing availability arable land; (3) required reductions extensive and ongoing environmental damage caused either poor agricultural practices or agriculture expansion, and; (4) increasingly unfavorable (duration severity) cultivation conditions accompany man-made climate change, driven ever-expanding urbanization its associated industrial practices. Mounting studies are repeatedly highlighting critical importance linking genotypes agronomically beneficial phenotypes and/or using a molecular approach help address this crisis, as “simply” clearing remaining natural ecosystems globe additional, non-modified crops not efficient, nor practice sustainable. The majority production sourced from small number members Poaceae family grasses, namely; maize (Zea mays L.), wheat (Triticum aestivum L.) rice (Oryza sativa L.). It is, therefore, significant concern all three these grass species susceptible range abiotic stresses, including drought salt stress. Highly conserved monocotyledonous dicotyledonous plant species, microRNAs (miRNAs) now well-established master regulators gene expression, influencing aspects development, mediating defense responses against pathogens adaptation Here we investigate variation in abundance profiles six known stress-responsive miRNAs, following exposure stress across key well compare those obtained genetic model Arabidopsis thaliana (L.) Heynh. Additionally, outline variables most likely primary contributors instances differential miRNA assessed exposure, specifically; identifying variations experimental methodology used assess abundance, distribution regulatory transcription factor binding sites within putative promoter region MICRORNA (MIR) encodes highly conserved, miRNA. We also discuss emerging role non-conserved, species-specific miRNAs play plant’s response