Split drive killer-rescue provides a novel threshold-dependent gene drive.

摘要: A wide range of gene drive mechanisms have been proposed that are predicted to increase in frequency within a population even when they deleterious individuals carrying them. This also allows associated desirable genetic material ("cargo genes") frequency. Gene drives garnered much attention for their potential use against globally important problems including vector borne disease, crop pests and invasive species. Here we propose novel mechanism could be engineered using combination toxin-antidote CRISPR components, each which already being developed other purposes. Population genetics mathematical models here demonstrate the threshold-dependent nature system its robustness imperfect homing, incomplete penetrance toxins transgene fitness costs, practical significance given real-world components inevitably such imperfections. We show although end-joining repair may cause break down, under certain conditions, it should persist over time scales relevant control programs. The provide localised suppression via sex ratio distortion or female-specific lethality is explored. Additionally, investigate effect on introduction thresholds adding an extra base element, showing this either decrease dependent parameter context.