An Assessment of Phase Transitions in Soybean Membranes

作者: Sharman D. O'Neill , A. Carl Leopold

DOI: 10.1104/PP.70.5.1405

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摘要: Phase transitions were measured in vesicles of phospholipids, alone and various combinations, pelleted mitochondrial membranes, using thermal (DSC) optical methods. The objective was to consider their possible involvement chilling injury soybeans (Glycine max [L.] Merr. cv Wayne 1977). Saturated phospholipids showed clear the temperature range 50 degrees C near 0 C. When mixtures two examined, there a marked lowering broadening transition peaks, shift temperatures intermediate temperatures. unsaturated that occur naturally no detectable phase this range, or combinations. Examination polar lipids from soybean asolectin revealed biological range; additions cations such as Ca(2+) La(3+) did not evoke them. Mitochondrial membrane pellets likewise transitions. application these direct methods examination components without addition foreign agents support suggested occurrence bulk which could be related soybeans.

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