ATR contributes to telomere maintenance in human cells
作者： Gaëlle Pennarun , Françoise Hoffschir , Deborah Revaud , Christine Granotier , Laurent R. Gauthier
DOI: 10.1093/NAR/GKP1248
关键词:
摘要: Telomere maintenance is essential to preserve genomic stability and involves several telomere-specific proteins as well DNA replication repair proteins. The kinase ATR, which has a crucial function in maintaining genome integrity from yeast human, been shown be involved telomere eukaryotic organisms, including yeast, Arabidopsis Drosophila. However, its role mammals remains poorly explored. Here, we report by using telomere-fluorescence situ hybridization (Telo-FISH) on metaphase chromosomes that ATR deficiency causes instability both primary human fibroblasts Seckel syndrome patients HeLa cells. aberrations resulting (i.e. sister fusions chromatid-type aberrations) are mainly generated during and/or after replication, involve leading lagging strand telomeres chromosome orientation-FISH (CO-FISH). Moreover, show strongly sensitizes cells the G-quadruplex ligand 360A, enhancing involving specifically telomeres. Altogether, these data reveal plays critical
oup.com
oxfordjournals.org
paperity.org
researchgate.net 参考文章(58)
T. de Lange, Shelterin: the protein complex that shapes and safeguards human telomeres Genes & Development. ,vol. 19, pp. 2100- 2110 ,(2005) , 10.1101/GAD.1346005
Hongyan Wang, Huichen Wang, Simon N Powell, George Iliakis, Ya Wang, None, ATR affecting cell radiosensitivity is dependent on homologous recombination repair but independent of nonhomologous end joining. Cancer Research. ,vol. 64, pp. 7139- 7143 ,(2004) , 10.1158/0008-5472.CAN-04-1289
Agnel Sfeir, Settapong T. Kosiyatrakul, Dirk Hockemeyer, Sheila L. MacRae, Jan Karlseder, Carl L. Schildkraut, Titia de Lange, Mammalian Telomeres Resemble Fragile Sites and Require TRF1 for Efficient Replication Cell. ,vol. 138, pp. 90- 103 ,(2009) , 10.1016/J.CELL.2009.06.021
David Baltimore, Eric J. Brown, ATR disruption leads to chromosomal fragmentation and early embryonic lethality. Genes & Development. ,vol. 14, pp. 397- 402 ,(2000) , 10.1101/GAD.14.4.397
Gemma K. Alderton, Hans Joenje, Raymonda Varon, Anders D. Børglum, Penny A. Jeggo, Mark O'Driscoll, Seckel syndrome exhibits cellular features demonstrating defects in the ATR-signalling pathway Human Molecular Genetics. ,vol. 13, pp. 3127- 3138 ,(2004) , 10.1093/HMG/DDH335
S. M. Bailey, Strand-Specific Postreplicative Processing of Mammalian Telomeres Science. ,vol. 293, pp. 2462- 2465 ,(2001) , 10.1126/SCIENCE.1062560
Colette Philippe, Philippe Coullin, Alain Bernheim, Double hybridization signals on the chromatid revealed by telomeric probes. Chromosome Research. ,vol. 7, pp. 497- 499 ,(1999) , 10.1023/A:1009258115435
Helmut P. G. Seckel, Bird-Headed Dwarfs: Studies in Developmental Anthropology including Human Proportions ,(1959)
Taku Naito, Akira Matsuura, Fuyuki Ishikawa, Circular chromosome formation in a fission yeast mutant defective in two ATM homologues Nature Genetics. ,vol. 20, pp. 203- 206 ,(1998) , 10.1038/2517
I. Ben-Porath, J.A. DeCaprio, M.W. Brooks, Shuichi Kaneko, Seishi Murakami, S.A. Stewart, J.L. Currier, W.C. Hahn, E.Y. Yu, S. Khurts, Kenichi Masutomi, R.A. Weinberg, G.B. Mets, Telomerase maintains telomere structure in normal human cells Cancer Research Institute report. ,vol. 2003, pp. 11- ,(2006)