A combinatorial approach involving E. coli cytosine deaminase and 5-fluorocytosine-nanoparticles as an enzyme-prodrug therapeutic method for highly substrate selective in situ generation of 5-fluorouracil
作者： Himali Horo , Sini Porathoor , Ruchi Anand , Lal Mohan Kundu
DOI: 10.1016/J.JDDST.2020.101799
关键词:
摘要: Abstract Enzyme-prodrug combination is a recognized therapeutic strategy for the treatment of cancer cells. Cytosine deaminases (CD) are non-mammalian proteins perceived to convert prodrug 5-fluorocytosine (5-FC), into active antitumor agent 5-fluorouracil (5-FU). In this study, we have synthesized 5-FC as well 5-FU loaded chitosan-silver nanoparticles in green synthesis approach, with high yield. The size was ascertained be below 25 nm, thus proficient penetrating Investigation human breast carcinoma cell line MDA-MB-468 manifested potent cytotoxicity compared prodrug, nanoparticles. E. coli CD (ECD) effectively hydrolyzes but inert silver
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