Water-Transfer Slows Aging in Saccharomyces cerevisiae

摘要: Transferring Saccharomyces cerevisiae cells to water is known extend their lifespan. However, it unclear whether this lifespan extension due slowing the aging process or merely keeping old yeast alive. Here we show that in water-transferred yeast, toxicity of polyQ proteins decreased and biomarker 47Q aggregates at a reduced rate lesser extent. These beneficial effects water-transfer could not be reproduced by diluting growth medium depended on de novo protein synthesis proteasomes levels. Interestingly, found upon 27 are downregulated, 4 upregulated 81 change intracellular localization, hinting an active genetic program enabling extension. Furthermore, aging-related deterioration heat shock response (HSR), unfolded (UPR) endoplasmic reticulum-associated degradation (ERAD), was largely prevented as activities these proteostatic network pathways remained nearly robust young yeast. The characteristics actively maintained indicate extended outcome process.