Intratumoral heterogeneity in colorectal carcinoma: trucut sampling for DNA ploidy analysis.

摘要: Background. Solid tumors, such as colorectal carcinomas, consist of cell subpopulations that differ both genetically and in their clinical behavior. Many authors have examined kinetics DNA content tumors correlation to pathological variables with different results. The interpretation those results present some difficulties related tumor heterogeneity date are unsolved. Our study is based on a new method colon cancer sampling for determination. aim this work was reduce the risk incorrect evaluation due heterogeneity. Material Methods. eleven selected cases T3 carcinoma. Fresh surgical specimens from primary site were taken during surgery. For each case at least four samples using 23 gauge trucut outside serosa through lumen colon. stained according modified Feulgen measured by image analysis. Three parameters evaluated. index, ploidy proliferation level (considered sum elements corresponding S G2 phases). Results. One (9.1%) showed diploid pattern; out (36.4%) tetra/polyploid pattern six (54.5%) an aneuploid pattern. monoclonal (27.3%), one two aneuploid. Eight polyclonal (72.7%). Considering single specimen, seven sixty-eight (10.3%) inadequate because scanty material. Twenty-five sixty-one adequate (41%) thirty-six (59%) polyclonal. Five (three polyclonal) same clones all samples. remaining interregional variability. In eight (75%) multiple stem lines evident, analyzing only sample close serosa, while (25%) it necessary examine order see polyclonality lesion. When mucosa where analyzed, however, not enough show five cases. Proliferation varied greatly parts carcinoma did correlate which taken. Conclusion. study, we demonstrated differences may exist between superficial deep part neoplasia greater variability deeper layers. Using samplings, possible point majority populations serosa. conclusion, biopsy permits full thickness tumoral mass allows, few samples, evaluate stemlines tumor.