Molecular, chemical and biological stability of plants derived from artificial seeds of Centella asiatica (L.) Urban—An industrially important medicinal herb

摘要: a b s t r c Synthetic seed technology offers viable option for large scale multiplication and in vitro conservation of endangered elite plant genotypes with economy space, time, operational cost. In the present study an efficient method preparation, short-term conservation, plantlet regeneration from synthetic seeds Centella asiatica (L.) Urban, is described first time. Axillary buds/nodal segments excised multiple shoot cultures were encapsulated 4% (w/v) sodium alginate beads following complexation 75 mM calcium chloride solution. The kept on moist filter paper sealed petri plates stored at 25 ± 3 ◦ C temperature 200 days. thus conserved recorded more than 85% germination conversion frequency upon shifting onto hormone-free Murashige Skoog medium. rooted plantlets 85-90% establishment soil did not show any morphological variation when compared mother plants. genetic fidelity regenerated plants was further confirmed using random amplified polymorphic DNA (RAPD) analysis wherein 19 decamer primers produced total 60 distinct monomorphic bands. HPLC leaf extracts seed-derived progeny showed qualitative quantitative uni- formity terms four major bioactive centellosides namely, asiaticoside, madecassoside, asiatic acid, madecassic acid. depicted same magnitude wound healing activity excision model mice as that plant. relevance devel- oped synseed micro-cloning exchange selected germplasm C. without affecting chemical biological activities discussed. © 2014 Elsevier B.V. All rights reserved.