A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes.
作者： RAMAKRISHNA U. RAO , STEVEN A. WILLIAMS , MELINDA SUSAPU , GARY J. WEIL , REDA M. R. RAMZY
DOI: 10.4269/AJTMH.2006.74.826
关键词:
摘要: We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting Wuchereria bancrofti DNA in human blood mosquitoes. An assay based on detection of the W. "LDR" repeat sequence was more sensitive than an Wolbachia 16S rDNA. The LDR-based microfilarial dried membrane filters or filter paper. also compared PCR with conventional (C-PCR) mosquito samples collected endemic areas Egypt Papua New Guinea. Although two methods had comparable sensitivity filarial reference samples, C-PCR practice field samples. Other advantages include its high-throughput capacity decreased risk cross-contamination between test believe that has great potential as a tool monitoring progress large-scale filariasis elimination programs.
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