Antitumor efficacy of viral therapy using genetically engineered Newcastle disease virus [NDV(F3aa)-GFP] for peritoneally disseminated gastric cancer

摘要: Peritoneal dissemination is a common and fatal clinical manifestation of gastric cancer with few effective therapies available. Natural Newcastle disease virus (NDV) has been shown to be an oncolytic agent, recent advances now allow genetic manipulation this improve killing safety. This study was designed investigate the effectiveness genetically engineered NDV in treatment peritoneally disseminated carcinoma. mutant containing modified F cleavage site insertion enhanced green fluorescent protein (GFP), NDV(F3aa)-GFP, tested vitro against human cells by standard cytotoxicity at different multiplicities infection. To test NDV(F3aa)-GFP vivo peritoneal carcinomatosis tumor model, MKN-74 were injected intraperitoneally (IP) severe combined immunodeficient mice. Mice treated either once or multiple times after challenge. Effective found vitro. dose-related correlated viral replication. GFP expression good marker The also as antitumor therapy model that simulates disease. Half animals had no evidence Genetically [NDV(F3aa)-GFP] administered IP from xenograft without significant toxicity. These data provide further rationale for trials involving cancer.