Interaction of recombinant IL-1 and recombinant tumor necrosis factor in the induction of mouse acute phase proteins.

摘要: Recombinant mouse and human IL-1 (alpha beta forms), as well rTNF-alpha when administered in vivo, induced the production of acute phase reactants: serum amyloid P-component (SAP), C3, fibrinogen. The SAP response to all three rIL-1 proteins reached a maximum at dose 10(4) U/mouse, which corresponds 1 10 micrograms protein. vivo consisted 10-fold increase levels, 2-fold C3 3-fold fibrinogen concentration. By contrast, much smaller (AP) protein (4-fold SAP) vivo. Administration combination if rTNF resulted an AP that was additive for SAP, synergistic fibrinogen, but only same amount by alone. Both recombinant monokines new synthesis isolated hepatocytes vitro with optimal occurring either U rIL-1/ml per 2 x 10(5) or 10(-3) U/ml rTNF. hepatocyte magnitude intact mice; however, TNF approximately times more efficient vitro. A mixture suboptimal doses were combined amounts rTNF-alpha. Overall, findings indicate both directly trigger their effect probably accounts substantial portion these mice.