Differential interaction of the monoclonal antibody AE-1 with acetylcholinesterase oligomers and monomers from rabbit muscle microsomes, human brain and fetal bovine serum
作者： César Flores-Flores , Alejandro Martı́nez-Martı́nez , Francisco J Campoy , Urs Brodbeck , Cecilio J Vidal
DOI: 10.1016/S0304-3940(97)00902-6
关键词:
摘要: The monoclonal antibody AE-1 raised against acetylcholinesterase (AChE) from human erythrocytes (HE) is shown to react with active asymmetric and tetrameric AChE components rabbit muscle microsomes (RMM), forms brain (HB) or fetal bovine serum (FBS). However, it failed bind monomers RMM HB. results of Western blot revealed that the determinant for consisted a conformational domain, not primary sequence region, in subunit. recognized HE FBS dimers, but monomers. formation labile immunocomplexes between subunits may explain lack interaction non-erythrocyte sources.
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