Microwave-accelerated surface plasmon-coupled directional luminescence 2: A platform technology for ultra fast and sensitive target DNA detection in whole blood

摘要: The application of Microwave-Accelerated Surface Plasmon-Coupled Luminescence (MA-SPCL) to fast and sensitive DNA hybridization assays in buffer whole blood is presented. In this regard, a model assay whereby fluorophore-labeled target ssDNA specific human immunodeficiency, Hepatitis C (Hep C), probed by an anchor probe immobilized on thin gold films, driven completion within 1 min with microwave heating, as compared identical completed ≈ 4 h at room temperature. Finite-Difference Time-Domain calculations show that disks are preferentially heated around the edges creating temperature gradient along disks, which turn results larger influx complementary towards probe-modified surface. Thermal images platform during heating also provide additional information pattern cavity. Finally, effects low power ability re-hybridize complimentary surface allows multiple re-use demonstrated. MA-SPCL technique offers alternative approach current based detection technologies, especially when speed sensitivity required, such identification or even RNA-based diseases using samples affect health.