A novel transiently expressed, integral membrane protein linked to cell activation. Molecular cloning via the rapid degradation signal AUUUA.
作者： H.W. Gaugitsch , E.E. Prieschl , F Kalthoff , N.E. Huber , T Baumruker
DOI: 10.1016/S0021-9258(19)49906-7
关键词:
摘要: A novel cDNA clone termed E16 which codes for an integral membrane protein of 241 amino acids with six transmembrane domains was isolated from peripheral blood lymphocytes. The is 4000 base pairs in length and exhibits unusually long 3'-untranslated region about 3000 nucleotides. Its expression at the mRNA level closely linked to cellular activation division. In all myeloid lymphoid cells, as well primary lymphocytes blood, transcripts are rapidly induced degraded after stimulation. This pattern unusual resembles more kinetic seen protooncogenes lymphokines T cell system. isolation made possible by a approach especially designed selectively cDNAs exhibit such kinetic. It based on combination differential screening subtracted library subsequent hybridization resulting phages short oligonucleotide (5'-TAAATAAA-TAAATA-3'). complementary trimer rapid degradation signal (AUUUA) present single or reiterated motif many short-lived transcripts.
sci-hub.st 参考文章(33)
B G Hudson, M Kataja, J Wieslander, Characterization of the human Goodpasture antigen. Clinical and Experimental Immunology. ,vol. 69, pp. 332- ,(1987)
B G Hudson, S T Reeders, K Tryggvason, Type IV collagen: structure, gene organization, and role in human diseases. Molecular basis of Goodpasture and Alport syndromes and diffuse leiomyomatosis. Journal of Biological Chemistry. ,vol. 268, pp. 26033- 26036 ,(1993) , 10.1016/S0021-9258(19)74270-7
S. Gunwar, F. Ballester, R. Kalluri, J. Timoneda, A.M. Chonko, S.J. Edwards, M.E. Noelken, B.G. Hudson, Glomerular basement membrane. Identification of dimeric subunits of the noncollagenous domain (hexamer) of collagen IV and the Goodpasture antigen. Journal of Biological Chemistry. ,vol. 266, pp. 15318- 15324 ,(1991) , 10.1016/S0021-9258(18)98618-7
S. Gunwar, S.T. Reeders, K.C. Morrison, M. Mariyama, K.E. Ebner, M.E. Noelken, B.G. Hudson, Goodpasture syndrome. Localization of the epitope for the autoantibodies to the carboxyl-terminal region of the alpha 3(IV) chain of basement membrane collagen. Journal of Biological Chemistry. ,vol. 266, pp. 24018- 24024 ,(1991) , 10.1016/S0021-9258(18)54385-4
R J Butkowski, J P Langeveld, J Wieslander, J Hamilton, B G Hudson, Localization of the Goodpasture epitope to a novel chain of basement membrane collagen. Journal of Biological Chemistry. ,vol. 262, pp. 7874- 7877 ,(1987) , 10.1016/S0021-9258(18)47648-X
E.G. Neilson, R. Kalluri, M.J. Sun, S. Gunwar, T. Danoff, M. Mariyama, J.C. Myers, S.T. Reeders, B.G. Hudson, Specificity of Goodpasture autoantibodies for the recombinant noncollagenous domains of human type IV collagen. Journal of Biological Chemistry. ,vol. 268, pp. 8402- 8405 ,(1993) , 10.1016/S0021-9258(18)52887-8
J Saus, J Wieslander, J P Langeveld, S Quinones, B G Hudson, Identification of the Goodpasture antigen as the alpha 3(IV) chain of collagen IV. Journal of Biological Chemistry. ,vol. 263, pp. 13374- 13380 ,(1988) , 10.1016/S0021-9258(18)37714-7
Nicholas A. Kefalides, Nobuko Ohno, Curtis B. Wilson, Howard Fillit, John Zabriski, Joel Rosenbloom, Identification of antigenic epitopes in type IV collagen by use of synthetic peptides Kidney International. ,vol. 43, pp. 94- 100 ,(1993) , 10.1038/KI.1993.16
J. Wieslander, P. Bygren, D. Heinegard, Isolation of the specific glomerular basement membrane antigen involved in Goodpasture syndrome. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 1544- 1548 ,(1984) , 10.1073/PNAS.81.5.1544
Stanley C. Gill, Peter H. von Hippel, Calculation of protein extinction coefficients from amino acid sequence data Analytical Biochemistry. ,vol. 182, pp. 319- 326 ,(1989) , 10.1016/0003-2697(89)90602-7