Expression of Leptin Receptor and Effects of Leptin on Papillary Thyroid Carcinoma Cells

摘要: Background. Obesity has been hypothesized to contribute the aggressiveness of thyroid cancer through production abnormal levels serum adipokines. Leptin receptor (OB-R) expression also documented in papillary (PTC). Aim. In this translational study, we analyzed vitro effects leptin on growth and migration cells (TPC-1 K1), molecular mechanisms underlying leptin’s action, influence prolonged exposure cell response a protein kinase inhibitor lenvatinib. The OB-R mRNA were investigated vivo series aggressive PTCs divided into two groups based presence BRAF mutation. Results. TPC-1 K1 cells, treatment with (500 ng/ml for 96 h) resulted mild increase proliferation (about 20% over control only ) both lines. Immunoblot analysis revealed slight phosphorylation AKT, but no effect β-catenin phospho-ERK expressions. inhibitory lenvatinib viability lines not influenced by treatment. transcript (in fresh tissues) proteins formalin-fixed paraffin-embedded specimens) expressed all PTC tissues examined, significant differences between BRAF-mutated BRAF-wild-type tumors. Conclusions. These results demonstrate role mildly increasing phenotype without influencing action Further studies will clarify whether it is possible target OB-R, PTCs, as an adjuvant approach these malignancies.