Characterization of the Single‐Strand‐Specific Nuclease S1 Activity on Double‐Stranded Supercoiled Polyoma DNA
作者： Jacques-E. Germond , Volker M. Vogt , Bernhard Hirt
DOI: 10.1111/J.1432-1033.1974.TB03446.X
关键词:
摘要: The DNA of polyoma virus consists double-stranded supercoiled circular molecules. single-strand-specific nuclease S1 converts these molecules to unit length rods. conversion occurs in a two-step reaction, with cutting first one the two strands and then intact strand opposite this nick. Cleavage by is consequence supercoiling. containing random single-strand breaks introduced physical or enzymatic means are also cut nicks.
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