Endofin is a novel component in EGF/EGFR oncogenic signaling

摘要: AACR Centennial Conference: Translational Cancer Medicine-- Nov 4-8, 2007; Singapore B74 Epidermal Growth Factor Receptor (EGFR) is a known oncogene and malfunctioning of its receptor endocytosis and/or degradation may result in excessive signaling that could contribute to cancer transformation tumorigenesis. Previously, we have identified Endofin, an endosomal protein, as one the novel phosphoproteins from analysis EGF-induced phosphotyrosine proteome using cICAT-based LC-MS/MS method. Here demonstrated Endofin phosphorylation peaks at 30 minutes starts decrease after 1 hour. Further studies also showed localization endofin early endosomes essential for phosphorylation. This indicated by drastic treated cells with wortmaninn, which prevents through inhibition PI3P production. In addition, vivo assay FYVE mutant, C753S, resulted abolishment phosphorylation, further supporting notion crucial On other hand, immunofluorescence staining transfected wild-type site-mutants revealed not required localization. All these results, taken together, suggest maybe important function endsomes, such regulation EGFR endocytosis/degradation, viable strategy therapeutics.