Effectiveness of Microwave Sterilization on Three Hard Chairside Reline Resins

摘要: PURPOSE The aim of this study was to evaluate the effectiveness microwave irradiation sterilization on hard chairside reline resins. MATERIALS AND METHODS Specimens three resins (Kooliner, Tokuso Rebase, and Ufi Gel Hard) were fabricated subjected ethylene oxide sterilization. specimens then individually inoculated (10(7) cfu/mL) with Tryptic Soy Broth media containing one tested microorganisms (C albicans, S aureus, B subtilis, P aeruginosa). After 48 hours at 37 degrees C, samples vortexed for 1 minute allowed stand 9 minutes, followed by a short vortex resuspend any organisms present. inoculation, 40 each material immersed in 200 mL water 650 W 6 minutes. Forty non-irradiated used as positive controls. Replicate (25 microL) suspension plated dilutions 10(-3) 10(-6) plates selective appropriate organism. All incubated C hours. incubation, colonies counted, data statistically analyzed Kruskal-Wallis test. Twelve prepared SEM. RESULTS showed consistent all individual after irradiation. SEM examination indicated an alteration cell morphology CONCLUSION Microwave minutes proved be effective