Metformin improves the sensitivity of ovarian cancer cells to chemotherapeutic agents.

摘要: Ovarian cancer is a common tumor of the reproductive system, and primarily responds to cytoreductive surgery cisplatin (DDP)-based chemotherapy. However, chemoresistance results in high ovarian mortality. Therefore, aim present study was investigate effects metformin on apoptosis autophagy drug-resistant SKOV3/DDP cells. To do so, MTT assay, flow cytometry, electron microscopy western blotting were used study. Metformin could inhibit growth SKOV3 cells concentration- time-dependent manner (P<0.05). The half-inhibitory concentration (IC50) values DDP methotrexate (MTX) 14.35 4.21 µg/ml for cells, 70.26 15.27 respectively. In addition, resistance index MTX 4.89 3.62, After combining with MTX, IC50 11.20 2.80 µg/ml, 6.21 2.74 decreased by 11.31- 6.18-fold. This indicated that cell proliferation inhibited when treated combination chemotherapeutic agents, compared agents alone. not observed cells; however, it following incubation 10 mmol/l 48 h. Furthermore, expression levels microtubule-associated protein 1 light chain 3-II upregulated These demonstrated can sensitize may be associated induction autophagy.