Differences in the time course of the metabolic response of B and non-B pancreatic islet cells to D-glucose and metabolized or non-metabolized hexose esters.

摘要: Abstract The early (min ≤ 1) and late 45) changes in NAD(P)H fluorescence caused by α-D-glucose pentaacetate, β-L-glucose β-D-galactose pentaacetate (1.7 mM each), alone or together with either L-leucine (10.0 mM) D-glucose (8.3 mM), were monitored purified pancreatic B non-B rat islet cells. Whilst a rapid increase the signal B-cells, but not so cells, two other monosaccharide esters, rapidly augmented both After 45 min, was increased cells pentaacetate. At this time, also exposed to L-leucine. These findings emphasize relevance of differences time course uptake as determinant redox state. They provide further support for role intracellular Ca 2+ regulating activity key -responsive mitochondrial dehydrogenases. Last, they reinforce view that effects hexose pentaacetates upon insulin glucagon release entail dual modality, linked catabolism their moiety direct effect esters themselves stereospecific receptor system.