Species Detection within the Echinococcus granulosus sensu lato Complex by Novel Probe-Based Real-Time PCRs

摘要: Infections with eggs of Echinococcus granulosus sensu lato (s.l.) can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion viable eggs, oncospheres hatch from the subsequently develop into fluid-filled larval cysts, most frequently liver or lungs. The slowly growing cysts progressively interfere organ function. risk infection is determined by range parasite, its pathogenicity other epidemiologically relevant parameters, which differ significantly among five species within E. s.l. complex. It therefore essential to diagnose correct help understand specific disease epidemiology facilitate effective implementation control measures. For this purpose, simple, fast cost-effective typing techniques are needed. We developed quantitative real-time polymerase chain reactions (qPCRs) target polymorphic regions mitochondrial genome In a single-step approach, we distinguished members four subgroups. These were stricto, equinus, ortleppi canadensis cluster. technique also allowed identification differentiation these Taenia taxa for samples isolated faeces.