Analysis of the aconitine alkaloids in traditional Chinese medicines by nonaqueous capillary electrophoresis using a new recording mode.

摘要: The determination of three aconitine alkaloids (hypaconitine, aconitine, mesaconitine) in five traditional Chinese medicines including two Tibetan medicines, Chuanwu, Caowu, Fuzi, Aconituin Tanguticum Maxim and Aconitum Gymnandrum by non-aqueous capillary electrophoresis using a new recording mode is described. dissociation constants mesaconitine hypaconitine have also been determined CZE were 7.71, 6.60 6.25, respectively. separation was achieved optimizing the applied voltage, pH concentration buffer. electrophoretic medium 20 mm borax-70% (v/v) methanol (pH 8.5) an uncoated (50 cm x 75 pm W.) used. Detection carried out with UV monitor at 214 rim. total time for under 13 min. Copyright (C) 2004 John Wiley Sons, Ltd.