[13c]-Constant-Time [15n,1h]-Trosy-Hnca for Sequential Assignments of Large Proteins
作者： Michael Salzmann , Konstantin Pervushin , Gerhard Wider , Hans Senn , Kurt Wüthrich
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摘要: The greatly improved sensitivity resulting from the use of TROSY during 15N evolution and amide proton acquisition enables recording HNCA spectra large proteins with constant-time 13Cα evolution. In [13C]-ct-[15N,1H]-TROSY-HNCA experiments a 2H/13C/15N-labeled 110 kDa protein, 7,8-dihydroneopterin aldolase Staphylococcus aureus, nearly all correlation peaks seen in [15N,1H]-TROSY-HNCA spectrum were also detected. resolution 13C dimension then enabled significant number sequential assignments that could not be obtained without [13C]-constant-time period.
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