Synthetic nanostructured wollastonite: Composition, structure and “in vitro” biocompatibility investigation

摘要: Abstract This article describes an original sol-gel (template) method for the synthesis of a dispersed form nanostructured wollastonite (CaSiO3), its gold nanoparticles (40–60 nm) functionalized CaSiO3/Au-NPs, and composite in composition with 20 mass% hydroxyapatite CaSiO3/HAp synthetic hydroxyapatite, that are widely used medicine reconstruction regeneration bone defects. The provides formation porous silica carcass samples average pore size 100–160 nm due to application polymer siloxane-acrylate as pore-forming template. authors studied processes template destruction phase using TGA/DTA/DSC (thermogravimetric analysis/differential thermal scanning calorimetry), XRD (X-ray diffraction analysis). methods SEM (scanning electron microscopy), low-temperature nitrogen adsorption, mercury porosimetry were identify morphology study structural characteristics (Sspec Vpor, distribution) based on hydroxyapatite. An “in vitro” model was functional activity (metabolism cytokine production) innate immune cells (neutrophils macrophages) contact obtained evaluate viability cells, ATPase activity, number cationic proteins, apoptosis cells. is novel these systems contributes existing knowledge biocompatibility vivo” model, which complex evaluation quality biomaterials grafts.