A PCR-based Strategy for Simple and Rapid Detection of Lactobacillus spp. in Content from Duodenum, Jejunum, Ileum and Cecum of Broiler Chickens

摘要: The gastrointestinal microflora is known to affect the health and performance of broilers. Prevention intestinal epithelium from pathogenic bacteria invasion by Lactobacillus spp. strains improvement broilers has been reported. This analyzed use bacteriological culture methods. These approaches have problems such as low sensitivities, inability detect non-cultivatable unknown species, time-consuming aspects, levels reproducibility. goal this study was develop a molecular method allowing rapid detection Lactohucillus at segments different ages. Thirty six Commercial were raised ages 4, 14 30 days, eight birds randomly sacrificed. Contents duodenum, jejunum, ileum cecum prepared their DNA extracted. specific based on PCR amplification 16S rRNA gene using oligonucleotide primers LAA-f LAA-r which product size 286 bp. For total intestine segments, 77 samples positive for naturally occurring laciobacillus method. value represented an incidence 80.21% with respect gut samples. Furthermore, 65.62, 90.62 84.37% days respectively. Meanwhile anterior exhibited nearly higher compared posterior segments. presented in manuscript developed routine, careful large number daily basis.