Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR/Cas9 system

作者: R.J. Bevacqua , R. Fernandez-Martín , V. Savy , N.G. Canel , M.I. Gismondi

DOI: 10.1016/J.THERIOGENOLOGY.2016.06.010

关键词:

摘要: … transfection with a vector harboring an antibiotic resistance cassette, cloning to rejuvenate the … The high rates of gene editing observed in our work support the notion that CRISPR/Cas9 …

参考文章(73)
Jingwei Wei, Stefan Wagner, Dan Lu, Paul Maclean, Daniel F. Carlson, Scott C. Fahrenkrug, Götz Laible, Efficient introgression of allelic variants by embryo-mediated editing of the bovine genome. Scientific Reports. ,vol. 5, pp. 11735- 11735 ,(2015) , 10.1038/SREP11735
Masahiro Sato, Miyu Koriyama, Satoshi Watanabe, Masato Ohtsuka, Takayuki Sakurai, Emi Inada, Issei Saitoh, Shingo Nakamura, Kazuchika Miyoshi, Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations. International Journal of Molecular Sciences. ,vol. 16, pp. 17838- 17856 ,(2015) , 10.3390/IJMS160817838
M. Crispo, A. P. Mulet, L. Tesson, N. Barrera, F. Cuadro, P. C. dos Santos-Neto, T. H. Nguyen, A. Crénéguy, L. Brusselle, I. Anegón, A. Menchaca, Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes PLOS ONE. ,vol. 10, pp. e0136690- ,(2015) , 10.1371/JOURNAL.PONE.0136690
Rotem Sorek, Victor Kunin, Philip Hugenholtz, CRISPR — a widespread system that provides acquired resistance against phages in bacteria and archaea Nature Reviews Microbiology. ,vol. 6, pp. 181- 186 ,(2008) , 10.1038/NRMICRO1793
Tang Hai, Fei Teng, Runfa Guo, Wei Li, Qi Zhou, One-step generation of knockout pigs by zygote injection of CRISPR/Cas system Cell Research. ,vol. 24, pp. 372- 375 ,(2014) , 10.1038/CR.2014.11
Yong Wang, Yinan Du, Bin Shen, Xiaoyang Zhou, Jian Li, Yu Liu, Jianying Wang, Jiankui Zhou, Bian Hu, Nannan Kang, Jimin Gao, Liqing Yu, Xingxu Huang, Hong Wei, Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA Scientific Reports. ,vol. 5, pp. 8256- 8256 ,(2015) , 10.1038/SREP08256
D. F. Carlson, W. Tan, S. G. Lillico, D. Stverakova, C. Proudfoot, M. Christian, D. F. Voytas, C. R. Long, C. B. A. Whitelaw, S. C. Fahrenkrug, Efficient TALEN-mediated gene knockout in livestock Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 17382- 17387 ,(2012) , 10.1073/PNAS.1211446109
Yuyu Niu, Bin Shen, Yiqiang Cui, Yongchang Chen, Jianying Wang, Lei Wang, Yu Kang, Xiaoyang Zhao, Wei Si, Wei Li, Andy Peng Xiang, Jiankui Zhou, Xuejiang Guo, Ye Bi, Chenyang Si, Bian Hu, Guoying Dong, Hong Wang, Zuomin Zhou, Tianqing Li, Tao Tan, Xiuqiong Pu, Fang Wang, Shaohui Ji, Qi Zhou, Xingxu Huang, Weizhi Ji, Jiahao Sha, Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos Cell. ,vol. 156, pp. 836- 843 ,(2014) , 10.1016/J.CELL.2014.01.027
Hui Yang, Haoyi Wang, Rudolf Jaenisch, Generating genetically modified mice using CRISPR/Cas-mediated genome engineering Nature Protocols. ,vol. 9, pp. 1956- 1968 ,(2014) , 10.1038/NPROT.2014.134
Hansruedi Büeler, Marek Fischer, Yolande Lang, Horst Bluethmann, Hans-Peter Lipp, Stephen J DeArmond, Stanley B Prusiner, Michel Aguet, Charles Weissmann, None, Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein Nature. ,vol. 356, pp. 577- 582 ,(1992) , 10.1038/356577A0