Detection and characterization of a functional complex of human immunodeficiency virus type 1 integrase and its DNA substrate by UV cross-linking.
作者： T Yoshinaga , Y Kimura-Ohtani , T Fujiwara
DOI: 10.1128/JVI.68.9.5690-5697.1994
关键词:
摘要: To analyze the association of human immunodeficiency virus type 1 integrase (IN) protein with DNA substrates, we applied a shortwave UV cross-linking method to in vitro integration system. Three photoadduct bands were detected on sodium dodecyl sulfate-polyacrylamide gels. The appearances these photoadducts examined various substrates and under several incubation conditions. Our data suggest that one is derived from sequence- strand-specific bound state early phase reaction before 3' processing reaction. We also show most complexes are competent for even after irradiation.
sci-hub.st 参考文章(38)
D.C. van Gent, C. Vink, A.A. Groeneger, R.H. Plasterk, Complementation between HIV integrase proteins mutated in different domains. The EMBO Journal. ,vol. 12, pp. 3261- 3267 ,(1993) , 10.1002/J.1460-2075.1993.TB05995.X
A. Engelman, F.D. Bushman, R. Craigie, Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. The EMBO Journal. ,vol. 12, pp. 3269- 3275 ,(1993) , 10.1002/J.1460-2075.1993.TB05996.X
D.J. Hazuda, A.L. Wolfe, J.C. Hastings, H.L. Robbins, P.L. Graham, R.L. LaFemina, E.A. Emini, Viral long terminal repeat substrate binding characteristics of the human immunodeficiency virus type 1 integrase. Journal of Biological Chemistry. ,vol. 269, pp. 3999- 4004 ,(1994) , 10.1016/S0021-9258(17)41733-9
M J Roth, P Schwartzberg, N Tanese, S P Goff, Analysis of mutations in the integration function of Moloney murine leukemia virus: effects on DNA binding and cutting. Journal of Virology. ,vol. 64, pp. 4709- 4717 ,(1990) , 10.1128/JVI.64.10.4709-4717.1990
F D Bushman, R Craigie, Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro. Journal of Virology. ,vol. 64, pp. 5645- 5648 ,(1990) , 10.1128/JVI.64.11.5645-5648.1990
P A Krogstad, J J Champoux, Sequence-specific binding of DNA by the Moloney murine leukemia virus integrase protein. Journal of Virology. ,vol. 64, pp. 2796- 2801 ,(1990) , 10.1128/JVI.64.6.2796-2801.1990
C Vink, D C van Gent, Y Elgersma, R H Plasterk, Human immunodeficiency virus integrase protein requires a subterminal position of its viral DNA recognition sequence for efficient cleavage. Journal of Virology. ,vol. 65, pp. 4636- 4644 ,(1991) , 10.1128/JVI.65.9.4636-4644.1991
J E Murphy, S P Goff, A mutation at one end of Moloney murine leukemia virus DNA blocks cleavage of both ends by the viral integrase in vivo. Journal of Virology. ,vol. 66, pp. 5092- 5095 ,(1992) , 10.1128/JVI.66.8.5092-5095.1992
M Katzman, R A Katz, A M Skalka, J Leis, The avian retroviral integration protein cleaves the terminal sequences of linear viral DNA at the in vivo sites of integration. Journal of Virology. ,vol. 63, pp. 5319- 5327 ,(1989) , 10.1128/JVI.63.12.5319-5327.1989
Tapan K. Misra, Duane P. Grandgenett, J. Thomas Parsons, Avian Retrovirus pp32 DNA-Binding Protein I. Recognition of Specific Sequences on Retrovirus DNA Terminal Repeats Journal of Virology. ,vol. 44, pp. 330- 343 ,(1982) , 10.1128/JVI.44.1.330-343.1982