Murine supraspinatus tendon injury model to identify the cellular origins of rotator cuff healing.
作者： Ryu Yoshida , Farhang Alaee , Felix Dyrna , Mark S. Kronenberg , Peter Maye
DOI: 10.1080/03008207.2016.1189910
关键词:
摘要: ABSTRACTPurpose of this study: To elucidate the origin cell populations that contribute to rotator cuff healing, we developed a mouse surgical model where full-thickness, central detachment is created in supraspinatus. Materials and methods: Three different inducible Cre transgenic mice with Ai9-tdTomato reporter expression (PRG4-9, αSMA-9, AGC-9) were used label shoulder. The defect was surgically injected tamoxifen at surgery cells sacrificed 1, 2, 5 weeks postoperatively. Frozen sections fluorescently imaged then stained Toluidine Blue re-imaged. Results: notable changes apparent (1) A long thin layer tissue formed on bursal side overlying supraspinatus tendon. (2) tendon proximal initially became hypercellular disorganized. (3) distal stump insertion underwent minimal remodeling. In uninjured...
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