Gamma irradiated Py/PVA for GOx immobilization on tapered optical fiber for glucose biosensing
作者： Sarada Idris , Nur Hidayah Azeman , Noor Azwen Noor Azmy , Chantara Thevy Ratnam , Mohd Adzir Mahdi
DOI: 10.1016/J.SNB.2018.07.053
关键词:
摘要: Abstract Tapered optical fiber coated with pyrrole/poly(vinyl alcohol)-glucose oxidase (Py/PVA-GOx) for glucose biosensing was successfully fabricated by radiation immobilization of GOx onto polymeric surfaces. Polymerization pyrrole and PVA crosslinking carried out means gamma irradiation. The nature enzyme studied Fourier transform infrared spectroscopy, X-ray photoelectron scanning electron microscopy atomic force microscopy. observation an absorption band at 1650 cm−1 binding energy formed 287.5 eV confirm the occurrence on polymer matrix. An increase in film thickness is observed after irradiation, which confirms entrapment into Py/PVA peak to valley roughness irradiated Py/PVA-GOx reveals intermolecular interaction between polymers enzyme. These characteristics are linked enzymatic reaction towards concentration. kinetic property a very low value obtained Michaelis-Menten constant, contributes improved adhesion fiber. response sensitivity were recorded as
sci-hub.se 参考文章(35)
Xiuyun Wang, Shunichi Uchiyam, Polymers for Biosensors Construction InTech. ,(2013) , 10.5772/54428
J KUMAR, S DSOUZA, Preparation of PVA membrane for immobilization of GOD for glucose biosensor. Talanta. ,vol. 75, pp. 183- 188 ,(2007) , 10.1016/J.TALANTA.2007.10.048
Yali Li, K. G. Neoh, Lian Cen, E. T. Kang, Porous and electrically conductive polypyrrole−poly(vinyl alcohol) composite and its applications as a biomaterial Langmuir. ,vol. 21, pp. 10702- 10709 ,(2005) , 10.1021/LA0514314
Mehmet Şenel, Simple method for preparing glucose biosensor based on in-situ polypyrrole cross-linked chitosan/glucose oxidase/gold bionanocomposite film. Materials Science and Engineering: C. ,vol. 48, pp. 287- 293 ,(2015) , 10.1016/J.MSEC.2014.12.020
D. Dey, T. Goswami, Optical Biosensors: A Revolution Towards Quantum Nanoscale Electronics Device Fabrication BioMed Research International. ,vol. 2011, pp. 348218- 348218 ,(2011) , 10.1155/2011/348218
G.G. Guilbault, G.J. Lubrano, An enzyme electrode for the amperometric determination of glucose Analytica Chimica Acta. ,vol. 64, pp. 439- 455 ,(1973) , 10.1016/S0003-2670(01)82476-4
HW Qiu, SC Xu, SZ Jiang, Z Li, PX Chen, SS Gao, C Zhang, DJ Feng, None, A novel graphene-based tapered optical fiber sensor for glucose detection Applied Surface Science. ,vol. 329, pp. 390- 395 ,(2015) , 10.1016/J.APSUSC.2014.12.093
M. V. Zeller, S. J. Hahn, The correlation of chemical structure and electrical conductivity in polypyrrole films by x‐ray photoelectron spectroscopy Surface and Interface Analysis. ,vol. 11, pp. 327- 334 ,(1988) , 10.1002/SIA.740110610
Francesco Dondero, Luciana Piacentini, Francesco Marsano, Mauro Rebelo, Laura Vergani, Paola Venier, Aldo Viarengo, Gene transcription profiling in pollutant exposed mussels (Mytilus spp.) using a new low-density oligonucleotide microarray. Gene. ,vol. 376, pp. 24- 36 ,(2006) , 10.1016/J.GENE.2006.02.015
Urszula Guzik, Katarzyna Hupert-Kocurek, Danuta Wojcieszyńska, Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases Molecules. ,vol. 19, pp. 8995- 9018 ,(2014) , 10.3390/MOLECULES19078995