Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli.

摘要: Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda nalidixic acid characterized. Both found grow cocci with increased doubling times. They resistance some mutagens (methylmethanesulfonate, ultraviolet light, gamma rays), antibiotics (nalidixic acid, ampicillin), phages (lambda, T6), sublethal heat hypotonic shock, decreased neutral detergents (sodium dodecyl sulfate, sodium deoxycholate), a protein synthesis inhibitor (streptomycin), respiratory azide). The nature changes in cell parameters indicate fundamental alterations the envelope structure mutant cells. new be multiply carbohydrate negative nonmotile conformity similar previously isolated mutants. Studies revertants phi 80 cya$sup +$ transductants indicated that pleiotropic phenotype is related single mutational event at or locus (auth)