Gene structure of CYP2C8 and extrahepatic distribution of the human CYP2Cs.
作者： Theresa S. Klose , Joyce A. Blaisdell , Joyce A. Goldstein
DOI: 10.1002/(SICI)1099-0461(1999)13:6<289::AID-JBT1>3.0.CO;2-N
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摘要: Extrahepatic tissue distribution of the mRNAs for four human CYP2Cs (2C8, 2C9, 2C18, and 2C19) was examined in kidney, testes, adrenal gland, prostate, brain, uterus, mammary ovary, lung, duodenum. CYP2C were detected by RT-PCR using specific primers each individual CYP2C. CYP2C8 mRNA CYP2C9 CYP2C18 found only duodenum CYP2C19 Immunoblot analysis small intestinal microsomes both 2C9 2C19 proteins. In addition, genomic clones sequenced, long-distance PCR performed to determine complete gene structure. spanned a 31 kb region. Comparative 2.4 upstream region with revealed two previously unidentified transcription factors sites, C/EBP HPF-1, latter might be involved hepatic expression. Although has been shown phenobarbital inducible, neither barbiturate-responsive regulatory sequence (a Barbie box) nor phenobarbital-responsive enhancer module (PBREM) within analyzed.
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